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KMID : 0894520080120030267
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Development & Reproduction
2008 Volume.12 No. 3 p.267 ~ p.274
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Regulation of Blastocyst Differentiation by the Serial Exposure of Conconavalin A and PGE₂
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Cheon Yong-Pil
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Abstract
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Differentiation of blastocyst is critical step for implantation and is under the control of regulation factors originated from embryo or reproductive tracts. The sequential communication with those factors is suspected as critical events for differentiation. It has been suggested that intracellular signaling pathways activated by calcium is essential in differentiation of blastocyst. Previously, it was known that concanavalin A (Con A) increase the levels of free calcium in blastocyst stage. However, Con A can not accelerate the hatching, although heparin-binding epidermal growth factor-like growth factor (HB-EGF), a modulator of calcium level, accelerate the hatching of blastocyst. In this study, it was investigated whether Con A or prostaglandin E₂ (PGE₂) can modulate the differentiation of blastocyst. Con A accelerated the expansion of blastocyst in both 1 hr pulse treatment group and continuous treatment group. However, Con A significantly suppressed the hatching in both groups. The inhibition was significantly strong in continuous treatment group compared with 1 hr pulse treatment group. On the other hand, PGE2 induced the increase the free calcium level, but did not accelerate the expansion. In addition 10 ㎛ PGE₂ inhibited hatching. However, PGE₂ could accelerate hatching in Con A pretreated blastocyst. PGE₂ also caused the increase of free calcium level in Con A pretreated blastocyst. From these results, it is suggested that changes of the free calcium level induce a different calcium-mediated signaling pathways. In addition, sequential stimulation by signal molecules may triggers the cellular mechanisms for the differentiation of blastocyst.
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KEYWORD
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Free calcium ion, Concanavalin A, PGE₂, Hatching
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